Program


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Deadline is 21 June 2021

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10x Genomics Lunch Symposium

Wednesday, 30 June 2021, 13:00 - 14:00 h

Redefine immunology with multi omic single cell and spatial characterization

Christophe Fleury has been working as Science and technology advisor for 10x genomics for 2 years. He has expertise in genomics with special emphasis on transcriptomics, bulk and single cell, obtained from diverse technical roles that he held at 10x Genomics and at Illumina prior to that and from over 10 years’ spent in academia. As a Science and technology advisor, Christophe puts his expertise to the service of customers to help them choose the best option for their projects and inform them on new products and developments.

 

Single-cell multi-omics analysis of the immune response in COVID-19
To investigate the immune response of COVID-19, we analysed individual blood cells of 130 patients from three medical centres in the UK. We revealed several new insights to COVID-19 pathogenesis whilst providing a valuable resource, exploitable for translational studies. We also highlighted the changes to cellular components during severe disease that could be targeted for therapy.

Emily Stephenson is Research Assistant who is also working towards her PhD. She plays an integral role for all ongoing projects in the Haniffa lab, using her specialty in single cell multi-omic technologies to generate single cell libraries for sequencing. She also works as a point of contact and collaborator with various other projects tied to the Haniffa lab all across the country. Her primary interest is in studying the developing human immune system during prenatal life as well as infection and inflammation. She does this by applying her skills in molecular biology and genomics that she honed by achieving her Bachelor’s Degree in Biology at Newcastle University and previous laboratory roles. Emily acts as a major coordinator for many aspects of the laboratory area, and enjoys using her experience to train junior researchers in her field. She is always happy to volunteer time to assist her colleagues, whether that’s discussing research possibilities or even using her skills as a trained phlebotomist!

 

Fluidigm Lunch Symposium

Thursday, 1 July 2021, 13:00 - 14:00 h

Immunological memory of SARS-CoV-2 specific antibodies and B cell responses at 12 months post SARS-CoV-2 infection
A better understanding of the immune response dynamics associated with SARS-CoV-2 infection and the protection against variants of concern (VOC) is critical for guiding vaccination campaigns and for the development of 2nd generation vaccines. To address these issues, we have monitored the dynamics of SARS-CoV-2 specific antibody response, both binding and neutralizing antibodies as well as memory B cells in patients with severe Covid-19 requiring hospitalization and in patients with mild disease (not-hospitalized) (total individuals n=114) during 12 months since the diagnosis of infection.

Alessandra Noto is an immunologist with extensive experience in adaptive cell biology during viral infections. Her recent research work involved the analysis of high-dimensional mass cytometry datasets of lymph node mononuclear cells which ultimately identified novel disease-associated targets. In particular, she identified candidates to boost efficient maturation of germinal center B cells for the generation of high affinity antibodies against HIV and identified novel markers to target latently HIV-1 infected CD4 T cells. More recently, she has contributed with the development of assays to study the immunological memory of SARS-CoV-2 in individuals who recovered from severe COVID-19 or mild SARS-CoV-2 infection and identified factors that correlated with the specific immune response.

 

Thermo Fisher Scientific Lunch Symposium

Friday, 2 July 2021, 13:00 - 14:00 h

Introducing the Invitrogen™ Attune™ CytPix™ Flow Cytometer: “Seeing is Believing”

Introducing a new standard in benchtop flow cytometry that helps effortlessly verify flow cytometry data by producing high-resolution images of user-defined cell populations. The Invitrogen Attune Cytpix flow cytometer is a flow cytometer that will collect up to 14 channels of fluorescent data while simultaneously capturing high resolution bright field images. Scientists can collect flow cytometry data at high-speed event rates without having to modify their standard panels and/or sample preparation. Collection of selected images at flow rates of up to 1000 µL per minute significantly increases the depth of flow cytometry data yielding important information for quality control, single cell morphological cell characterization, and cell-to-cell interactions. “Seeing is believing” is especially true in experiments where having confirmation of a few specific cells can determine the final outcome of the experiment. To aid scientists in controlling the mountains of data storage that are required for typical imaging flow cytometers, the imaging-enhanced flow cytometer includes tools to customize the data footprint to their experiments. Scientist can collect only the images that are of interest. By combining new camera technologies for rapid image capture with our acoustic focusing technology to address depth of field limitations, the Attune CytPix flow cytometer enables Thermo Fisher Scientific to enable researchers to use this first hybrid system compatible with high speed, high throughput flow cytometry and high-speed imaging.

The Bigfoot Spectral Cell Sorter: Accelerating Science

In May 2020, the Bigfoot Spectral Cell Sorter was shared with the global flow cytometry community with a virtual launch. Since then, there have been many systems placed in laboratories and users of all types have been able to immerse themselves into the Bigfoot experience and explore the various capabilities and unique features that it offers. In this tutorial, Bigfoot user share her experiences using the instrument, diving into the versatility of a full spectrum sorter, ease of use for both seasoned and new users, and the cutting edge capabilities of doing high parameter spectral sorts.

Automated Isolation of Functional T-cells

Here we have optimized an automated cell isolation approach on the KingFisher instrument to increase flexibility, sample throughput, reduce hands-on time and reduce personal error rate to make every experiment count. The automated cell isolation is exemplified by CD4+/CD8+ T cell isolation, followed by a smooth transfer to T-cell Activation, using instrumentation from Thermo Fisher Scientific for handling and analysis (KingFisher™, Floyd™ and Attune™). Removing the beads from the isolated cells provided functional T-cells ready to be activated and expanded by Dynabeads™ CD3/CD28.

Becton Dickinson Lunch Symposium

Saturday, 3 July 2021, 13:00 - 14:00 h

Fate challenge purges the Treg repertoire of a heterogeneous, unstable subset

Susan Schlenner is Assistant Professor at the KU Leuven, Belgium, where she heads the Adaptive Immunity lab. Her research is focused on mechanisms underlying Treg (in)stability and the heterogeneity of the unstable Treg population. More recently, she initiated research to explore the role of RNA modifications in T cells. Her research involves the generation and use of transgenic mice, advanced molecular and cellular technologies and multi-omics approaches. Her expertise in genome engineering and flow cytometry led her to co-found the KUL genome engineering platform and to take on the directorship of the KUL Flow and Mass Cytometry core facility. She further is a co-founder of the Leuven Institute for Single Cell Omics (LISCO).